PBMC Cryopreservation Medium

For the cryopreservation of PB- and CB-derived mononuclear cells and buffy coats
Ready-to-use

GMP-manufactured with pharmacopeia ingredients

Chemically Defined

Protein-free

High-density, high-viability

Identity of PBMC Cryopreservation Protocol Medium

Cryopreservation Protocol for PBMC Freezing Media
Collect the mononuclear cells from peripheral blood or cord blood,centrifuge the cell (500×g,5minutes,RT) and remove the supernatant.

Resuspend cell pellet with proper solution (saline,culture medium or DPBS) ,followed by cell counting and cell survival rate detection.A viability of over 90% before cryopreservation is preferred.Then centrifuge (500×g,5 minutes,RT) and remove the supernatant.

Resuspend cell pellet using the product to reach a cell density of 1×10⁶-1×108/mL and a freezing volume of 0.5-1.5 mL/tube.If using a cryopreservation bagforlarge volume cryopreservation,please contact the technical team to obtain the programed cooling curve.Users can also add autologous plasma,serum,or HSA according to experimental needs to improve post-thaw viability.

Transfer the cryovial to a-80℃ freezer.If long-term freezing is required,transfer to liquid nitrogen after 12hours.

Thawing Protocol for PBMC Medium
1. After removing from liquid nitrogen,immediately immerse the cryovial into a 37℃ water bath,stir to rapidly thaw the cells within 2 minutes.

2. Transfer thawed cells to a conical tube,slowly add 9 times volume of proper diluent.

3. Equilibrate by gently invert the tube for 10 times.

4. Centrifuge (500×g,5 minutes,RT) and remove the supernatant.

5. Resuspend cell pellet with proper media/solution.

6. Perform cell count test if needed.

7. Proceed for downstream application.

Viability Test for PBMC Cryopreservation Medium
Add thawed cells to 4 times the volume of complete culture medium,transfer to a centrifuge tube,and centrifuge for 5 minutes.Discard the supernatant,resuspend the cells using DPBS or complete culture medium,and then test the cell survival rate.

Ordering Information of PBMC Cryopreservation Media

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