Recombinant Kex2 Protease

CAS: 99676-46-7

Gene-Biocon Recombinant Kex2 is a genetically engineered protein expressed in Pichia pastoris and purified by high-pressure liquid chromatography. The optimal pH of Kex2 protease is 9.0, and the optimal temperature is 37 ℃. It is stable in buffer (pH 5.0-6.0).

Kex2 Protease Recommended Method
Recommended reaction buffer: pH 7.0-9.0, 50mM Tris-HCl, 2mM Ca2+ or HEPES, 5mM Ca2+. If it is not used immediately after dissolution, it is recommended to use 20mM (pH 5.2) NaAc- HAc, 2mM Ca2+ buffer to dissolve the lyophilized powder.
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During the reaction, use pH 7.0-9.0, 50mM Tris-HCl, 2mM Ca2+ or HEPES, 5mM Ca2+ as the reaction diluent.
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Kex2 Transport and Storage Stability
Transport stability: It is transported in an ice pack to keep it active. Storage stability: Store at -15°C below, stable within 24 months. Dissolve in storage buffer (20mM NaAC-HAC, 2mM Ca2+, pH5.2) and store in sub-packages -15ºC below stable within 6 months.

Notice and Disclaimer
For the specific optimal testing concentration, please refer to the relevant literature, or explore and optimize through experiments according to the purpose of the experiment
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The product is for research use only and should not be used for diagnosis or treatment of animals or humans.
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