UMI Adapter & UDI Primers

Overview
IGT® UMI Adapter & UDI Primers is a series of kits used to detect samples with low-frequency mutations. Unique molecular identifiers (UMIs) are a type of molecular barcoding that tag each molecule in the library, which can reduce the false-positive rate and increase the sensitivity of mutation detection. iGeneTech offers 64 different 6-bp ngs umi adapter with edit distance not less than 3, which is enough to distinguish between original molecules and correct sequencing errors. This makes the solution highly effective for UMI NGS applications in precision genomics.

iGeneTech provides 384 combinations of different UDI primers for high-throughput sequencing in a single lane that reduces the index contaminations due to index hopping.

iGeneTech also offers umi adapter & udi primers without UMI-UDI technology, giving researchers flexible options tailored to their sequencing workflows.

Advantages
High accuracy: 99.9% background noise filtered out by UMI correction

High sensitivity of detection: sensitivity and stable mutation detection of cfDNA samples with mutation rates as low as 0.02%

Reliable UMIs: Special non-random UMI design strategy with edit distance not less than 3

Low index cross-contamination rate: Unique Dual Index (UDI) primers included to filter the hopped reads.

Technical Principle
Illumina
MGI
Principle for data correction UMI adapter
Performance
High Accuracy and Sensitivity of Low-Frequency Mutation Detection
Test of low-frequency Horizon 0.1% cfDNA reference standard using IGT® UMI Adapter & UDI Primers successfully detected all 8 mutations with reliable mutation rate.

Significantly Reduce the False-Positive Rate
UMI can significantly reduce the false-positive rate by error correction. The following figure shows the raw data and corrected data with standard, SSCS and DSC de-duplication correction.

The DSC correction using UMI reduced the number of mutations with VAF less than 3% from 526 to 15, including all the 8 known mutations.

Ordering Info
UMI Adapter & UDI Primers
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